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Published on: April 11, 2016
A non-invasive QPCR method monitoring DNA based therapy of bladder cancer patients
Suhail Ayesh1, Rasha Abu-Lail, Abraham Hochberg
1The Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, and Molecular Genetics Laboratory, Makassed Islamic Charitable Hospital, Jerusalem, Israel. suhail@cc.Is.huji.ac.il
Abstract:
Real-time PCR technology is highly advantageous for gene studies based on the genetic nature of the transferred material. Urine and blood samples were collected before and after treatment. Treatment of bladder carcinoma patients with plasmid constructs expressing the diphtheria toxin gene was monitored. Detection range from 5x10(6) copies to
