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Determination of the binding affinities of plasmid DNA using fluorescent intercalators possessing an acridine
D Sabolová1, M Kozurková, P Kristian
1Department of Biochemistry, Institute of Chemistry, Faculty of Sciences, P.J. Safárik University, 041 54 Kosice, Slovakia. sabdan@kosice.upjs.sk
Abstract:
Novel acridine derivatives, wherein the steric factor has been varied systematically through substitution at the 9 position of the acridine ring, were evaluated as convenient fluorescent probes for nucleic acid detection. The binding affinities of N-(9-acridinylthiocarbamoyl)amino acids (ATA) with plasmid DNA (pUC 19) were investigated using UV-vis spectrophotometry, fluorometric titration and quantum chemical calculations (AM1). From spectrofluorometric analysis, the binding constants for the DNA-ATA complexes were determined. To elucidate its DNA intercalation, the most preferable tautomeric structure of ATA was established by means of AM1 calculations.
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