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Do-It-Yourself Device for Recovery of Cryopreserved Samples Accidentally Dropped into Cryogenic Storage Tanks
Published on: May 11, 2012
Probability of lethal damages of cryopreserved biological objects during storage
I P Vysekantsev1, T M Gurina, V F Martsenyuk
1Institute for problems of cryobiology and cryomedicine of the National Academy of Sciences of the Ukraine, 23 Pereyaslavskaya str., 61015, Kharkov, Ukraine. cryo@online.kharkov.ua
Abstract:
This study demonstrated that with a cyclic change of the temperature from -196 degree C up to -130 degree C or -100 degree C part of cryopreserved microorganisms, tumor and human embryo cells died. This should be taken into account when moving and transporting the cryopreserved objects. Storage of samples under the above-mentioned stable temperatures did not result in additional death of cells.

