Related Experiment Video
Updated: Aug 9, 2026
A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
Published on: February 20, 2012
[AFLP fingerprinting of Pinellia ternata and its application]
Juan Du1, Xiao-jun Ma, Xue-dong Li
1College of Life Sciences, Capital Normal University, Beijing, China.
Objective:
To obtain more information on DNA fingerprintings and to confirm the genetic relationships of ten wide populations of Pinellia tornata.
Method:
Collect wide-type or cultivated P. tornata from ten major production areas nation-wide as material. Twelve combinations of EcoR I and Mse I primers were used to asses divergence among ten populations. The data were analyzed using unweighted pair-group method, based on arithmetic averages (UPGMA) bootstrap analysis. Cluster analyses were performed by using NTSYSpc software version 2.1. The alkaloid was extracted from P. ternate with chlorolform and determined in UV absorption spectroscop.
Result:
1,673 bands were applied with 12 combinations. These results of cluster were congruent with differences in total alkloids.
Conclusion:
The results of the study showed a potential application of AFLP fingerprinting for identification of P. ternata.
More Related Videos
08:42Infection of In Vivo and In Vitro Pines with the Pinewood Nematode Bursaphelenchus xylophilus and Isolation of Induced Volatiles
Published on: September 27, 2024
09:39DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat (VNTR) - Fragment Length Analysis (FLA)
Published on: July 15, 2011