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[Determination of triterpene delta-amyrone in Sedum sarmentosum]
Su-ping Cao1, Li-fang Liu, Qiang Wang
1Department of Chinese Pharmceutical Analysis, China Pharmaceutical University, Nanjing.
Objective:
To establish the quantitative analysis method of triterpene delta-amyrone in Sedum sarmentosum by HPLC-ELSD.
Method:
The Zorbax Sil Column was used and the mobile phase was dichloromethane-methanol (99:1). The flow rate was set at 0.5 mL x min(-1), the temperature of drift tube was set at 100 degrees C and the nebulizer nitrogen flow rate was 3.0 L x min(-1).
Result:
The linear range of sigma-amyrone was 2.12-10.6 microg. The average recovery was 100.5%.
Conclusion:
The results showed that the method was reliable, simple and precise. It would be beneficial for the study of the quality control of S. sarmentosum.
