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Performance of the DNA-Citoliq liquid-based cytology system compared with conventional smears
V A F Alves1, A Castelo, A L Filho
1School of Medicine, São Paulo University, Brazil. venancio@uol.com.br
Objective:
To evaluate the performance of a new, manual, simplified liquid-based system, DNA-Citoliq (Digene Brasil), employed under routine conditions as compared to conventional smears collected from six collaborating private laboratories.
Methods:
A panel of cytopathologists, who served as the gold standard diagnosis, adjudicated discordant opinions.
Results:
Of 3206 pairs of slides considered valid for comparison, there were 3008 in full agreement (93.8%), 112 (3.5%) with one diagnostic category discrepancies, and 86 (2.7%) discordant cases. Among the 288 borderline+ by either method, DNA-Citoliq detected abnormalities in 243 (84.4%), and conventional smears (CS) detected abnormalities in 178 (61.8%) (McNemar test, P < 0.000), a 36.5% increased detection of borderline+ cases.
Conclusions:
For mild dyskaryosis, DNA-Citoliq detected 176 cases and CS 125 cases (McNemar test, P < 0.000); and for moderate+severe dyskaryosis 66 versus 32 cases respectively (McNemar test, P < 0.000).
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