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Related Experiment Videos

Leptin regulates olfactory-mediated behavior in ob/ob mice.

Thomas V Getchell1, Kevin Kwong, Christopher P Saunders

  • 1Department of Physiology, University of Kentucky, Lexington, KY 40536-0298, USA. tgetche@uky.edu

Physiology & Behavior
|March 22, 2006
PubMed
Summary

Leptin (ob/ob) and leptin receptor (db/db) mutant mice show impaired olfactory-driven food finding. Leptin replacement in ob/ob mice restored normal olfactory behavior, suggesting leptin

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Area of Science:

  • Neuroscience
  • Endocrinology
  • Behavioral Biology

Background:

  • Leptin is a key hormone regulating energy balance and appetite.
  • Mutations in leptin (ob/ob) or its receptor (db/db) lead to obesity and altered feeding behaviors.
  • The role of leptin in olfactory-mediated behaviors, particularly pre-ingestive responses, remains incompletely understood.

Purpose of the Study:

  • To investigate the impact of leptin deficiency and leptin receptor mutation on olfactory-mediated pre-ingestive behavior in mice.
  • To determine the efficacy of leptin replacement therapy in restoring normal olfactory-driven food-finding behavior in ob/ob mice.
  • To identify potential central or peripheral sites of leptin action within the olfactory system.

Main Methods:

  • Comparison of olfactory-mediated food-finding behavior in leptin-deficient (ob/ob) and leptin receptor-mutant (db/db) mice versus wild-type (wt) controls.

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  • Utilized a buried food paradigm with repeated testing over several days to assess food-finding times.
  • Administered leptin or carrier via intraperitoneal injection to ob/ob mice to evaluate the effects of leptin replacement.
  • Employed immunohistochemistry and in situ hybridization to localize leptin and its receptor expression in olfactory tissues.

    • Main Results:

    • ob/ob and db/db mice exhibited significantly faster food-finding times compared to wt mice, indicating altered olfactory-mediated behavior.
    • Leptin replacement in ob/ob mice at a high dose (5 microg/g) normalized food-finding times to levels comparable to wt mice.
    • Lower doses of leptin or carrier injection did not fully restore wild-type behavior in ob/ob mice.
    • Leptin and leptin receptor expression was detected in the olfactory and nasal mucosae, suggesting local sites of action.

    Conclusions:

    • Leptin plays a crucial role in modulating olfactory-mediated pre-ingestive behavior.
    • Leptin receptor signaling within the olfactory system is essential for normal food-finding responses.
    • Leptin replacement therapy can effectively restore olfactory-driven behaviors in leptin-deficient states.