Related Experiment Video
Updated: Aug 9, 2026

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
[Study on mitochondrial DNA genetic diversity of Tibetan antelope]
Hui Zhou1, Di-Qiang Li, Yu-Guang Zhang
1College of Animal Science and Technology of Hunan Agricultural University, Changsha 410128, China. zhouhui0402@163.com
Abstract:
With noninvasive DNA genotyping technology, we investigated genetic diversity of Tibetan antelope by analyzing mitochondrial DNA variationìthose samples are collected from Hoh Xil National Nature Reserve, Qinghai province, China. A total of 444-446 bp of the mitochondrial control region was sequenced form 10 individuals. The results showed that A% + T%(61.8%) was higher than C%+G% (38.2%) obviously. Ten haplotypes were identified in the 10 samples. The result showed 48 polymorphic sites after comparisons of the 10 haplotype samples, among them, including 44 transitions, 1 transversion, 1 insertion and 2 deletions. The average genetic distance of haplotypes is 0.031. The haplotypic diversity is 1.000. The nucleotide diversity is 0.0296. The average number of nucleotide differences is 13.127. It showed that the Tibetan antelope population has high variation in mitochondrial D-loop sequence.
Related Concept Videos
Animal Mitochondrial Genetics
Applications of Molecular Taxonomy
Modern Molecular Taxonomy
Evolutionary Relationships through Genome Comparisons
Diversity of Archaea I
Conservation of Small Populations

