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LTA recognition by bovine gammadelta T cells involves CD36
1Montana State University, Bozeman, MT 59718, USA.
Journal of Leukocyte Biology
|March 23, 2006
Summary
Bovine gammadelta T cells express CD36 protein, a molecule previously thought to be restricted to myeloid cells. This CD36 protein contributes to their response to microbial lipoteichoic acid (LTA).
Area of Science:
- Immunology
- Cell Biology
Background:
- CD36 facilitates monocyte Toll-like receptor 2 (TLR2) recognition of lipoteichoic acid (LTA).
- Bovine gammadelta T cells were previously found to express CD36 transcripts.
Purpose of the Study:
- To determine if bovine gammadelta T cells express CD36 protein.
- To investigate if bovine gammadelta T cell CD36 functions similarly to the monocyte molecule.
Main Methods:
- Detection of CD36 transcripts and protein in sorted bovine gammadelta T cells.
- Stimulation with phorbol 12-myristate 13-acetate (PMA)/ionomycin to assess changes in CD36 expression.
- Assessment of CD36 function using anti-CD36 monoclonal antibody in response to LTA.
Main Results:
- CD36 transcripts and protein were detected in resting and stimulated bovine gammadelta T cells.
- PMA/ionomycin treatment increased CD36 transcript and protein levels, with surface expression peaking at 72 hours.
- Anti-CD36 antibody inhibited macrophage-inflammatory protein-1alpha gene expression in LTA-activated gammadelta T cells.
Conclusions:
- Bovine gammadelta T cells express CD36 protein, expanding its known expression profile beyond myeloid and endothelial cells.
- CD36 on gammadelta T cells plays a role in their response to microbial LTA.