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Biologic and antigenic similarity of virus-induced migration inhibition factor to conventional, lymphocyte-derived
Abstract:
Macrophage migration inhibition factor (MIF) is one of a class of lymphocyte-derived mediator substances (lymphokines) which plays a role in the mechanism of cellular immunity. A variety of other soluble factors produced by non-lymphoid cells have been shown to have effects on macrophage mobility similar to that of MIF. In the present study we demonstrate that one such factor, (MIFV) derived from simian virus 40-infected kidney cells in culture, has several other properties in common with lymphocyte-derived MIF (MIFL), MIFV can be adsorbed on Sepharose bead columns conjugated with an antiserum prepared against MIFL, demonstrating at least some antigenic similarity. Moreover, MIFV can substitute for MIFL in an in vivo system involving the suppression of cutaneous manifestations of cellular immunity by intravenous injection of the lymphokine. These observations, taken in conjunction with the similarity of the in vitro effect of MIFV and MIFL, and their similar chromatographic behavior, suggest that MIFV and MIFL may be identical molecular species.
Insights
A novel factor derived from virus-infected kidney cells (MIFV) shows significant similarities to lymphocyte-derived macrophage migration inhibition factor (MIFL). This suggests MIFV and MIFL may be the same molecular entity, impacting cellular immunity.
Area of Science:
- Immunology
- Cell Biology
- Virology
Background:
- Macrophage migration inhibition factor (MIF) is crucial for cellular immunity.
- Non-lymphoid cells produce factors affecting macrophage mobility, similar to MIF.
Purpose of the Study:
- To investigate a factor (MIFV) from virus-infected kidney cells for similarities with lymphocyte-derived MIF (MIFL).
- To determine if MIFV and MIFL are identical molecular species.
Main Methods:
- Antigenic similarity assessed by adsorption to anti-MIFL antiserum-conjugated Sepharose beads.
- Functional similarity evaluated using an in vivo system suppressing cellular immunity.
- Chromatographic behavior compared between MIFV and MIFL.
Main Results:
- MIFV demonstrated antigenic similarity to MIFL.
- MIFV effectively substituted for MIFL in suppressing cutaneous cellular immunity in vivo.
- In vitro effects and chromatographic behavior of MIFV and MIFL were comparable.
Conclusions:
- MIFV shares key antigenic and functional properties with MIFL.
- Evidence strongly suggests MIFV and MIFL are identical molecular species.
- This finding has implications for understanding cellular immunity mechanisms.