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Updated: Aug 9, 2026

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling
Published on: November 17, 2019
A functional proteomics approach for the detection of nuclear proteins based on derepressed importin alpha
Erik Blazek1, Michael Meisterernst
1Department of Gene Expression, National Research Center for Environment and Health-GSF, Institute of Molecular Immunology, Munich, Germany.
Abstract:
The identification of functional proteomes is a major challenge in proteomic research. Here we describe a method for the detection and isolation of nuclear (localization sequence containing) proteins using a derepressed import receptor (DIRE) as a synthetic antibody. We demonstrate that the DIRE method specifically detects nuclear localization sequence containing proteins. Application to activation of primary T-lymphocytes exemplifies the potential use of DIRE for comparative proteomics and for diagnostics.
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