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Effects of retrorsine on mouse hepatocyte proliferation after liver injury
Xiao-Fei Zhou1, Qian Wang, Jian-Xin Chu
1Center for Developmental Biology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
Aim:
To study the effect of retrorsine on mouse hepatocyte proliferation.
Methods:
Mice and rats were treated respectively with two injections of retrorsine (as retrosine-treated group) or saline (as non-treated group) at 2 wk intervals. They received a single injection of carbon tetrachloride (CCl4) 4 wk later. On d 0, 1, 2, 3, 4, 6, 15 after CCl4 administration, the animals were killed and their livers were excised. Hematoxylin and eosin (HE) staining and Ki-67 antibody immunohistochemical analysis of liver samples were used to evaluate the pathological changes and hepatocyte proliferation.
Results:
In rats treated with retrorsine and CCl4, the liver displayed obvious megalocytosis, proliferation of mild bile duct,small hepatocyte-forming nodule, which were not found in liver samples from non-treated group. However,in mice treated with retrorsine combined with CCl4, the liver displayed hepatocyte degeneration and necrosis in perivenous areas.There was no obvious difference between retrorsine-treated group and non-treated group. Ki-67 immunohistochemical analysis showed that in rats treated with retrorsine, the positive hepatocytes mainly found in small hepatocyte nodules, were obviously less than those in non-treated group. The mice treated with retrorsine showed that the number of Ki-67 positive hepatocytes was very high and more than that in non-treated group.
Conclusion:
Retrorsine has no effect on mouse hepatocyte proliferation.
