Vaginal Lactobacillus isolates inhibit uropathogenic Escherichia coli

Area of Science:

• Microbiology
• Probiotics
• Urogenital Health

Background:

• Recurrent cystitis is often caused by uropathogenic Escherichia coli (UPEC).
• The vaginal microflora, particularly Lactobacillus species, plays a crucial role in maintaining urogenital health.
• Afa/Dr diffusely adhering E. coli (DAEC) strains are implicated in urinary tract infections.

Purpose of the Study:

• To investigate the antibacterial activities of specific Lactobacillus jensenii and Lactobacillus gasseri strains.
• To evaluate the efficacy of these Lactobacillus strains against pathogenic Afa/Dr DAEC strains.
• To explore the potential of these probiotics in combating recurrent urinary tract infections.

Main Methods:

• Isolation of Lactobacillus jensenii and Lactobacillus gasseri strains from healthy women's vaginal microflora.
• In vitro assessment of antibacterial activity against Afa/Dr DAEC E. coli strains IH11128 and 7372.
• Evaluation of Lactobacillus adhesion inhibition and internalization prevention in HeLa cell models.

Main Results:

• Selected Lactobacillus strains demonstrated significant inhibition of E. coli IH11128 and 7372 growth and viability.
• Adherent Lactobacillus strains effectively inhibited the adhesion of E. coli IH11128 to HeLa cells.
• Lactobacillus strains also reduced the internalization of E. coli IH11128 within HeLa cells.

Conclusions:

• Lactobacillus jensenii and Lactobacillus gasseri strains possess potent antibacterial properties against uropathogenic E. coli.
• These vaginal Lactobacillus strains can prevent E. coli adhesion and invasion, key factors in cystitis development.
• The findings support the potential use of these specific Lactobacillus strains as probiotics for managing and preventing recurrent urinary tract infections.