Adiponectin protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity
Tae Woo Jung1, Ji Young Lee, Wan Sub Shim
1The Brain Korea 21 Project for Medical Science, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Republic of Korea.
Abstract:
1-Methyl-4-phenylpyridinium ion (MPP+), an inhibitor of mitochondrial complex I, has been widely used as a neurotoxin because it elicits a severe Parkinson's disease-like syndrome characterized by elevation of intracellular reactive oxygen species level and apoptotic death. Adiponectin, secreted from adipose tissue, mediates systemic insulin sensitivity with liver and muscle as target organs. Adiponectin can also suppress superoxide generation in endothelial cells. In the present study, we investigated the protective effects of adiponectin on MPP+-induced cytotoxicity in human neuroblastoma SH-SY5Y cells, as well as the underlying mechanism. Our results suggest that the protective effects of adiponectin on MPP+-induced apoptosis may be ascribed to its anti-oxidative properties, anti-apoptotic activity via inducing expression of SOD and catalase, and regulation of Bcl-2 and Bax expression. These data indicated that adiponectin might provide a useful therapeutic strategy for the treatment of progressive neurodegenerative diseases such as Parkinson's disease.
Insights
Adiponectin protects against 1-Methyl-4-phenylpyridinium ion (MPP+)-induced neurotoxicity by reducing oxidative stress and apoptosis. This suggests adiponectin as a potential therapeutic for Parkinson's disease.
Area of Science:
- Neuroscience
- Endocrinology
- Cell Biology
Background:
- 1-Methyl-4-phenylpyridinium ion (MPP+) is a neurotoxin that induces Parkinson's disease-like symptoms by increasing reactive oxygen species and apoptosis.
- Adiponectin, an adipose-secreted hormone, regulates insulin sensitivity and exhibits anti-oxidative properties in endothelial cells.
Purpose of the Study:
- To investigate the protective effects of adiponectin against MPP+-induced cytotoxicity in human neuroblastoma SH-SY5Y cells.
- To elucidate the underlying mechanisms of adiponectin's protective action.
Main Methods:
- Utilized human neuroblastoma SH-SY5Y cells exposed to MPP+.
- Assessed the impact of adiponectin on cell viability, apoptosis, and the expression of key oxidative stress and apoptosis-related genes (SOD, catalase, Bcl-2, Bax).
Main Results:
- Adiponectin demonstrated significant protective effects against MPP+-induced cytotoxicity and apoptosis.
- Adiponectin treatment led to increased expression of superoxide dismutase (SOD) and catalase, indicating enhanced anti-oxidative capacity.
- Adiponectin modulated the expression of Bcl-2 and Bax, shifting the balance towards anti-apoptotic signaling.
Conclusions:
- Adiponectin exerts neuroprotective effects against MPP+-induced neurotoxicity through its anti-oxidative and anti-apoptotic properties.
- Adiponectin's ability to upregulate SOD and catalase, and regulate Bcl-2/Bax expression, underlies its therapeutic potential.
- Adiponectin represents a promising therapeutic strategy for neurodegenerative diseases like Parkinson's disease.
More Related Videos
19:44Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen
Published on: May 30, 2012
06:34New Application of an Atmospheric Pressure Plasma Jet as a Neuro-protective Agent Against Glucose Deprivation-induced Injury of SH-SY5Y Cells
Published on: October 9, 2017