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A selective differential medium for Lactobacillus plantarum
Carmen Bujalance1, Maria Jiménez-Valera, Encarnacion Moreno
1Department of Microbiology, Faculty of Pharmacy, University of Granada, Granada 18071, Spain.
Journal of Microbiological Methods
|March 24, 2006
Summary
A new selective medium, LPSM, effectively detects and quantifies exogenous Lactobacillus plantarum in fecal samples. This method aids in evaluating probiotic colonization by inhibiting other gut bacteria while allowing L. plantarum to grow and produce a distinct yellow color.
Area of Science:
- Microbiology
- Gastroenterology
- Probiotics
Background:
- Quantifying exogenous lactobacilli in fecal samples is crucial for assessing probiotic bacteria colonization in the gut.
- Existing methods may lack specificity or require complex enrichment steps.
Purpose of the Study:
- To develop a selective and differential medium for the specific culture and enumeration of exogenous Lactobacillus plantarum in fecal samples.
Main Methods:
- Development of LPSM medium containing ciprofloxacin to inhibit endogenous bacteria.
- Utilized sorbitol fermentation and bromocresol purple indicator for colorimetric detection of L. plantarum.
- Quantitative assays comparing LPSM sensitivity with existing media.
Main Results:
- LPSM demonstrated comparable sensitivity to enriched and Lactobacillus-adapted media for quantitative assays.
- Ciprofloxacin in LPSM effectively inhibited most intestinal bacteria, including endogenous lactic acid bacteria.
- Exogenous L. plantarum strains were successfully detected and enumerated via yellow color production due to sorbitol fermentation.
Conclusions:
- LPSM is a suitable and effective medium for the selective detection and enumeration of Lactobacillus plantarum in fecal samples.
- The developed medium aids in the evaluation of intestinal colonization by probiotic L. plantarum.