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Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
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Optimization of membrane protein overexpression and purification using GFP fusions

David Drew1, Mirjam Lerch, Edmund Kunji

  • 1Department of Biochemistry and Biophysics, Stockholm University, SE-106 91 Stockholm, Sweden.

Nature Methods
|March 24, 2006
PubMed
Abstract

No abstract available in PubMed .

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Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...