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Updated: Aug 9, 2026
A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
Published on: April 20, 2018
Differences between the fingerprints generated from total RNA and poly-A RNA using a modified procedure of cDNA-AFLP
Jian-Rong Guo1, F Schnieder, J A Verreet
1Institute of Phytopathology, Christian-Albrechts-University Kiel, Hermann-Rodewald-Str. 9, 24118 Kiel, Germany. jguo@phytomed.uni-kiel.de
Abstract:
A modified procedure of cDNA-amplified fragment length polymorphism (cDNA-AFLP) combined with silver staining is described which generates clear band patterns from both fungal total RNA and poly-A RNA. The silver staining procedure takes only 25 min. Some 42-55 transcript-derived fragments (TDFs) were yielded from the total RNA of eight fungal pathogens. In comparison with the fingerprints generated from Mycosphaerella graminicola total RNA, those from poly-A RNA showed eight TDFs with an increased intensity and also seven additional TDFs. It suggests that poly-A RNA is preferable as starting material for ideal cDNA-AFLP analysis.
