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Silicone based polyurethane materials: a promising biocompatible elastomeric formulation for cardiovascular
1Laboratory for Biomaterials & Graft Technology, G. Pasquinucci Hospital, Institute of Clinical Physiology CNR, Massa, Italy.
Journal of Materials Science. Materials in Medicine
|March 24, 2006
Summary
This study evaluated a new poly(ether)urethane and polydimethylsiloxane material for cardiovascular uses. Both in vitro and in vivo tests confirmed the material is non-toxic across all tested silicone concentrations.
Area of Science:
- Biomaterials Science
- Polymer Chemistry
- Cardiovascular Engineering
Background:
- Cardiovascular device development requires biocompatible materials.
- Poly(ether)urethane (PEtU) and polydimethylsiloxane (PDMS) blends offer tunable properties.
- Manufacturing processes can impact material biocompatibility.
Purpose of the Study:
- To assess the biocompatibility of a novel PEtU-PDMS composite material.
- To evaluate potential cytotoxicity introduced during material preparation and manufacturing.
- To determine the influence of varying PDMS concentrations on material safety.
Main Methods:
- A PEtU-PDMS composite was fabricated into porous membranes via spray deposition.
- In vitro cytotoxicity assays used human umbilical vein endothelial cells (HUVECs) and L929 mouse fibroblasts.
- In vivo studies were conducted using the same material compositions as in vitro tests.
Main Results:
- In vitro tests included light microscopy, MTT reduction, neutral red uptake, and BrdU incorporation assays.
- No significant cytotoxicity was observed in HUVECs or L929 cells exposed to material extracts.
- In vivo results corroborated in vitro findings, indicating no adverse effects.
- Material preparation and manufacturing procedures did not introduce toxicity at any PDMS concentration.
Conclusions:
- The PEtU-PDMS composite material demonstrates excellent biocompatibility for cardiovascular applications.
- The spray-deposition technique and material formulation are safe and do not induce cytotoxicity.
- This novel biomaterial is suitable for further development in cardiovascular devices.