Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Neutrophil chemotactic factors.

L Harvath1

  • 1Division of Hematology, Food and Drug Administration, Bethesda, MD 20892.

EXS
|January 1, 1991
PubMed
Summary

Neutrophils use chemotaxis to migrate to inflammation sites, guided by various chemoattractants. Modifications to these chemoattractants significantly impact neutrophil migration, a key process in inflammation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

FDA's proposed regulatory approach to regulation of hematopoietic stem/progenitor cell products.

Cytotherapy·2010
Same author

National Heart, Lung, and Blood Institute of the National Institutes of Health forum on immune reconstitution and cellular therapy following hematopoietic stem-cell transplantation.

Cytotherapy·2002
Same author

Food and Drug Administration's proposed approach to regulation of hematopoietic stem/progenitor cell products for therapeutic use.

Transfusion medicine reviews·2000
Same author

Overview of fluorescence analysis with the confocal microscope.

Methods in molecular biology (Clifton, N.J.)·1999
Same author

Assay for phagocytosis.

Methods in molecular biology (Clifton, N.J.)·1999
Same author

Assay for filamentous actin.

Methods in molecular biology (Clifton, N.J.)·1999

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Polymorphonuclear leukocytes (neutrophils) are crucial immune cells involved in inflammatory responses.
  • Neutrophil recruitment to inflammatory sites is mediated by chemoattractants, which stimulate directed migration (chemotaxis).
  • Chemoattractants can be endogenous (e.g., LTB4, C5a) or exogenous (e.g., bacterial N-formyl peptides).

Purpose of the Study:

  • To review well-defined neutrophil chemotactic factors.
  • To explore the structure-function relationships of these chemotactic factors.
  • To understand how chemoattractant properties are altered by modifications.

Main Methods:

  • Review of existing literature on neutrophil chemoattractants.
  • Analysis of structure-function relationships of peptide chemoattractants.
  • Examination of the impact of oxidative modification and amino acid sequence changes.

Main Results:

  • Chemoattractants are diverse, originating from host cells, enzymatic cleavage, or bacterial invasion.
  • Oxidative modification of methionine residues or alterations in amino acid sequence significantly change chemoattractive properties.
  • Specific examples of chemoattractants include neutrophil activating proteins, LTB4, platelet activating factor, C5a, and N-formyl peptides.

Conclusions:

  • Neutrophil chemotaxis is a complex process regulated by a variety of chemoattractants.
  • The chemical structure of chemoattractants is critical for their function.
  • Understanding these factors and their modifications is key to understanding inflammatory processes.

Related Experiment Videos