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Detection of Functional Matrix Metalloproteinases by Zymography
Published on: November 8, 2010
Inactivation of human plasma C1-inhibitor by human PMN leucocyte matrix metalloproteinases
V Knäuper1, S Triebel, H Reinke
1University of Bielefeld, Faculty of Chemistry, Department of Biochemistry, Germany.
FEBS Letters
|September 23, 1991
Abstract:
Highly purified human polymorphonuclear (PMN) leucocyte matrix metalloproteinases, collagenase and gelatinase, cleaved human plasma C1-inhibitor at the carboxyl site of Ala439 (P6). This led to a concomitant loss of C1-inhibitor activity. An additional cleavage site, at the carboxyl site of Ser441 (P4), was observed during PMN leucocyte gelatinase-induced inactivation, and a minor fragment of the plasma C1-inhibitor was generated.

