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Updated: Aug 9, 2026
Isolated Lung Perfusion System in the Rabbit Model
Published on: July 15, 2021
In-vitro 1H-MRS of excised rabbit lung: evaluation of preservation method of the lung using 10% DMSO solution
Hajime Yokota1, Jianfei Guo, Munetaka Matoba
1Department of Radiology, Kanazawa Medical University, Ishikawa 920-0293, Japan.
Purpose:
This study was performed to investigate the lactate, creatine, and choline changes in excised rabbit lung metabolites using proton magnetic resonance spectroscopy (1H-MRS).
Materials And Methods:
Rabbit lung was preserved with -20 degrees C 10% dimethyl sulfoxide (DMSO) solution until used, and the lactate, creatine, and choline levels of the rabbit lung were evaluated by 1H-MRS (6.34T superconducting unit). The control of lung tissue was excised under low-temperature conditions of liquid nitrogen, and preserved in 10% DMSO solution at -20 degrees C until evaluated. The lung tissues were excised at room temperature, and 30 min later, the tissues were saved in 10% DMSO solution at -20 degrees C until evaluated, and the lactate, creatine, and choline were measured by 1H-MRS after 20, 40, 60 and 80 days.
Results:
The lactate, creatine, and choline in the control tissue measured 0.412 micromol/g, 0.024 micromol/g and 0.696 micromol/g, respectively. The lactate, creatine, and choline in the experimental tissue measured 0.414 micromol/g, 0.029 micromol/g and 0.388 micromol/g after 60 days, respectively.
Conclusion:
In this study, the level of lactate within tissues preserved in 10% DMSO solution at -20 degrees C achieved a state of equilibrium within 60 days.
