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Updated: Aug 9, 2026

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High-Resolution Comparison of Bacterial Conjugation Frequencies
Published on: January 10, 2019
Conjugational transfer kinetics of pLS20 between Bacillus subtilis in liquid medium
Mitsuhiro Itaya1, Nagayoshi Sakaya, Satoko Matsunaga
1Mitsubishi Kagaku Institute of Life Sciences, Tokyo, Japan. mita2001@sfc.keio.ac.jp
Bioscience, Biotechnology, and Biochemistry
|March 25, 2006
Abstract:
pLS20-mediated conjugational transfer between Bacillus subtilis was investigated not on conventional solid media but in liquid culture. Detailed conjugational kinetics revealed that pLS20 transmission occurred at a limited cellular growth stage of both donor and recipient. Mutation of the recipient recA did not significantly interfere with the conjugational transfer process.

