Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Resistance factor-mediated spectinomycin resistance.

D H Smith1, J A Janjigian, N Prescott

  • 1Department of Pediatrics, Harvard Medical School and Children's Hospital Medical Center, Boston, Massachusetts 02115.

Infection and Immunity
|January 1, 1970
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of periconceptional nutrition on the growth, behaviour and survival of the neonatal lamb.

Animal reproduction science·2015
Same author

Predicting the risk of Clostridium difficile infection following an outpatient visit: development and external validation of a pragmatic, prognostic risk score.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2015
Same author

Observation of entanglement-dependent two-particle holonomic phase.

Physical review letters·2014
Same author

Analysis of solution residues by glow discharge mass spectrometry.

Journal of the American Society for Mass Spectrometry·2013
Same author

Secular trends in kidney disease: is the decreased incidence of renal replacement therapy due to a decrease in chronic kidney disease incidence?

Clinical nephrology·2008
Same author

Harnessing stakeholder perspectives to improve the care of osteoporosis after a fracture.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2008

A study found that 51% of drug-resistant enteric bacteria were resistant to spectinomycin (Spc). Many contained transferable R factors, suggesting a single enzyme mediates resistance to multiple antibiotics.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Enteric bacteria are a significant source of antibiotic resistance.
  • Spectinomycin (Spc) resistance is a growing concern in clinical settings.
  • Transferable R factors play a crucial role in the dissemination of antibiotic resistance.

Purpose of the Study:

  • To investigate the prevalence and mechanisms of spectinomycin (Spc) resistance in natural isolates of enteric bacteria.
  • To characterize the R factors mediating Spc resistance and their associated resistance profiles.
  • To elucidate the enzymatic basis for Spc resistance and cross-resistance to other aminoglycosides.

Main Methods:

  • Screening of 100 natural enteric bacterial isolates for spectinomycin (Spc) resistance.

Related Experiment Videos

  • Plasmid isolation and characterization of transferable R factors.
  • In vitro enzymatic assays using bacterial extracts to study adenylation of Spc and related compounds.
  • Genetic analysis to confirm the role of specific genes in resistance.

    • Main Results:

    • 51% of isolates exhibited resistance to spectinomycin (Spc).
    • 46% harbored transferable R factors conferring Spc resistance.
    • All Spc-resistant (Spc(R)) R factors also conferred resistance to streptomycin and bluensomycin and were of the fi(+) type.
    • Bacterial extracts demonstrated in vitro adenylation of Spc, dihydrospectinomycin, actinamine, streptomycin, and bluensomycin in the presence of ATP and Mg(++).

    Conclusions:

    • A single enzyme likely mediates the adenylation of spectinomycin (Spc) and related aminoglycosides, explaining the observed cross-resistance.
    • Transferable R factors are a significant mechanism for the spread of Spc resistance in enteric bacteria.
    • Understanding these resistance mechanisms is crucial for developing strategies to combat antibiotic resistance.