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Cytopathic Effects and Ultrastructure of Mycoplasma agalactiae var. bovis (Donetta strain)

R S Hirth1, M E Tourtellotte, S W Nielsen

  • 1Department of Animal Diseases, University of Connecticut, Storrs, Connecticut 06268.

Infection and Immunity
|July 1, 1970
PubMed

Insights

This study examined the Donetta strain mycoplasma using electron microscopy, revealing significant pleomorphism and extracellular locations near host cells. Serum is crucial for mycoplasma multiplication and cytopathic effects, though the exact cause remains undetermined.

Area of Science:

  • Microbiology
  • Cell Biology
  • Electron Microscopy

Background:

  • Mycoplasma species are known pathogens causing various diseases.
  • The Donetta strain, tentatively identified as Mycoplasma agalactiae var. bovis, requires detailed characterization.
  • Understanding mycoplasma-host interactions is crucial for disease management.

Purpose of the Study:

  • To characterize the morphology and cellular interactions of the Donetta strain mycoplasma.
  • To investigate the factors influencing mycoplasma replication and cytopathic effect (CPE) in cell cultures.

Main Methods:

  • Electron microscopy was employed to examine mycoplasma morphology in broth and cell cultures.
  • Mycoplasma-host cell interactions were observed in vitro.
  • The role of serum in mycoplasma multiplication and CPE induction was assessed.

Main Results:

  • The Donetta strain exhibited significant pleomorphism, including membrane-lined spaces and invaginations.
  • Mycoplasmas were primarily extracellular, closely associated with host cell membranes.
  • Serum was essential for both mycoplasma multiplication and the development of CPE.
  • Cytoplasmic invasion was not identified as the primary cause of CPE.

Conclusions:

  • The Donetta strain mycoplasma displays unique morphological characteristics.
  • Mycoplasma-host cell interactions occur predominantly extracellularly.
  • Serum is a critical factor for Donetta strain mycoplasma pathogenesis and replication.

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