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Chemotactic activity generated by staphylococcal enterotoxin B.

C P Craig1, S J Normann, V McGann

  • 1U. S. Army Medical Research Institute of Infectious Diseases, Frederick, Maryland 21701.

Infection and Immunity
|January 1, 1971
PubMed
Summary
This summary is machine-generated.

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Staphylococcal enterotoxin B (SEB) triggers a heat-labile, antibody-sensitive factor that attracts human polymorphonuclear leukocytes (PMN). This suggests a role for PMN in SEB-induced enterotoxemia.

Area of Science:

  • Immunology
  • Toxicology
  • Microbiology

Background:

  • Staphylococcal enterotoxin B (SEB) exhibits significant toxicity via oral and parenteral routes in mammals.
  • Understanding the mechanisms of SEB toxicity is crucial for developing effective countermeasures.

Purpose of the Study:

  • To investigate the generation of chemotactic factors for human polymorphonuclear leukocytes (PMN) induced by SEB.
  • To characterize the properties of the SEB-induced chemotactic factor(s).

Main Methods:

  • Incubation of purified SEB with fresh human and rabbit serum at 37°C.
  • Assaying for chemotactic activity using human PMN and rabbit peritoneal PMN.
  • Heat inactivation studies (56°C for 30 min) and antibody inhibition assays.

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Main Results:

  • SEB incubation with normal serum generated a factor(s) chemotactic for human PMN.
  • Similar chemotactic activation was observed using rabbit serum and autologous peritoneal PMN.
  • The generated chemotactic activity was heat-labile and inhibited by precipitating antibody to SEB.

Conclusions:

  • SEB induces the formation of a heat-labile, antibody-sensitive chemotactic factor(s).
  • This factor differs from endotoxin-generated chemotactic factors.
  • The findings suggest a mechanism involving PMN in the pathophysiology of staphylococcal enterotoxemia.