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Related Experiment Videos

Sensitization of complement-resistant smooth gram-negative bacterial strains.

B L Reynolds1, H Pruul

  • 1Department of Microbiology, University of Adelaide, Adelaide, South Australia 5000.

Infection and Immunity
|March 1, 1971
PubMed
Summary

Smooth gram-negative bacteria resistance to complement can be overcome by altering environmental conditions. Specific cation concentrations can increase serum sensitivity, suggesting lipopolysaccharide layer disorganization.

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Area of Science:

  • Microbiology
  • Immunology
  • Bacteriology

Background:

  • Gram-negative bacteria exhibit variable susceptibility to complement-mediated bactericidal activity.
  • Rough strains are typically serum-sensitive, while smooth strains are generally serum-resistant.
  • Serum resistance in smooth strains is often attributed to their lipopolysaccharide (LPS) outer layer.

Purpose of the Study:

  • To investigate the influence of environmental conditions on the serum sensitivity of smooth gram-negative bacteria.
  • To explore mechanisms by which environmental factors can modulate bacterial resistance to complement.
  • To determine if serum resistance of smooth strains can be reversed under specific laboratory conditions.

Main Methods:

  • Testing serum sensitivity of smooth gram-negative organisms under varying environmental conditions.

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  • Utilizing immune serum, complement, divalent cations, univalent cations, and ethylenediaminetetraacetic acid (EDTA).
  • Observing changes in bacterial susceptibility to bactericidal action.

    • Main Results:

    • Smooth gram-negative organisms, typically resistant to complement, demonstrated increased sensitivity in specific environments.
    • High concentrations of certain univalent cations significantly enhanced serum sensitivity.
    • Ethylenediaminetetraacetic acid (EDTA) potentiated the effect of univalent cations on serum sensitivity.

    Conclusions:

    • Environmental conditions can significantly alter the serum sensitivity of smooth gram-negative bacteria.
    • The lipopolysaccharide layer's protective role may be overcome by manipulating cation concentrations.
    • Environmental factors may induce structural disorganization of the smooth lipopolysaccharide, leading to increased susceptibility to complement-mediated killing.