Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Staphylococcus aureus Protoplasting Induced by d-Cycloserine.

R Virgilio1, C Gonzalez, N Muñoz

  • 1Departamento de Microbiologia, Facultad de Química y Farmacia, Universidad de Chile, Santiago, Chile.

Journal of Bacteriology
|December 1, 1970
PubMed
Summary

Sublethal d-cycloserine doses cause Staphylococcus aureus cell walls to rupture equatorially, releasing protoplasts. This finding offers insights into bacterial cell wall dynamics and potential therapeutic targets.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Longitudinal stress fractures of the tibia: report of three cases.

British journal of rheumatology·1997
Same author

Cellular aspects of vascular remodeling in hypertension revealed by confocal microscopy.

Hypertension (Dallas, Tex. : 1979)·1997
Same author

Carbon monoxide inhibits hypoxic pulmonary vasoconstriction in rats by a cGMP-independent mechanism.

Pflugers Archiv : European journal of physiology·1997
Same author

Acute renal failure and flank pain after binge drinking and non-steroidal anti-inflammatory drugs.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association·1997
Same author

Osteoarticular and muscle infectious lesions in patients with the human immunodeficiency virus.

Clinical rheumatology·1997
Same author

Methods in Drosophila cell cycle biology.

Current topics in developmental biology·1997

Area of Science:

  • Microbiology
  • Bacterial Cell Wall Biology
  • Antibiotic Research

Background:

  • Staphylococcus aureus is a significant human pathogen.
  • Understanding bacterial cell wall structure is crucial for developing new antimicrobial strategies.
  • Sublethal antibiotic concentrations can reveal novel mechanisms of bacterial response.

Purpose of the Study:

  • To investigate the effect of sublethal d-cycloserine on Staphylococcus aureus cell wall integrity.
  • To characterize the morphological changes induced by d-cycloserine exposure.
  • To determine the mechanism of protoplast formation in d-cycloserine-treated Staphylococcus aureus.

Main Methods:

  • Growing Staphylococcus aureus cells were exposed to sublethal concentrations of d-cycloserine.

Related Experiment Videos

  • Microscopic techniques were employed to observe cellular morphology and cell wall structure.
  • Analysis focused on identifying specific sites of cell wall rupture.

    • Main Results:

    • Addition of sublethal d-cycloserine doses induced cell wall rupture in growing Staphylococcus aureus.
    • Rupture occurred specifically along an equatorial ring.
    • This equatorial rupture led to the liberation of protoplasts.

    Conclusions:

    • Sublethal d-cycloserine disrupts Staphylococcus aureus cell wall synthesis at the equator.
    • The equatorial rupture mechanism provides a novel pathway for protoplast formation.
    • This highlights d-cycloserine's potential in targeting bacterial cell wall integrity.