Alternative mRNAs encoding the alpha 1b-adrenergic receptor are expressed in a tissue-dependent manner in the...

Probing total cellular and poly [A+] RNA isolated from various rat tissues with a full-length cDNA encoding the hamster alpha 1-adrenergic receptor results in detection of two transcripts, 3.3 kb and 2.7 kb, which probably both encode the alpha 1b-adrenergic receptor subtype. Both the 3.3 kb and 2.7 kb mRNAs were found to be associated with hepatic polysomes which suggests that these mRNA species are translated into protein. Using non-overlapping 5' and 3' cDNA probes, large sequence differences were not evident between the 3.3 kb and 2.7 kb mRNAs, although the 3'-probe hybridized to a 4.0 kb mRNA in addition to the two smaller transcripts in poly [A+] RNA isolated from renal cortex, but not other tissues. The relative amounts of the 3.3 kb and 2.7 kb mRNAs varied considerably among the five tissues studied. However, the ratio of the two transcripts remained relatively constant in the same tissue taken from animals at different developmental ages. Currently, the physiological significance of multiple alpha 1b-adrenergic receptor gene transcripts is unclear. However, our results suggest that alpha 1b-adrenergic receptor gene expression in the rat is under complex regulatory control that in part is tissue-dependent.