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Updated: Jul 23, 2026

Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy
Published on: January 18, 2018
Transcription factor AP-1 modulates the activity of the human foamy virus long terminal repeat
B Maurer1, E Serfling, V ter Meulen
1Institut für Virologie und Immunbiologie, Universität Würzburg, Germany.
Abstract:
The human foamy virus (HFV) contains within the U3 region of its long terminal repeat (LTR) three perfect consensus sequences for the binding of the inducible transcription factor AP-1. Results of DNase I footprint protection and gel retardation assays demonstrated that proteins in extracts of HeLa and BHK-21 cells as well as bacterially expressed Jun and Fos proteins bind to these AP-1 sites. By conducting transient expression assays using chloramphenicol acetyltransferase plasmids carrying LTR sequences with point-mutated AP-1 sites, it was found that the three AP-1 sites contribute to the optimal activity of the HFV promoter. It is shown that induction of the HFV LTR by 12-O-tetradecanoylphorbol-13-acetate (TPA) and serum factors is mediated through the AP-1 sites.
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