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CHARACTERISTICS OF BRUCELLAPHAGE.

C R McDuff1, L M Jones, J B Wilson

  • 1Department of Bacteriology, University of Wisconsin, Madison, Wisconsin.

Journal of Bacteriology
|February 1, 1962
PubMed
Summary
This summary is machine-generated.

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Epidemiology and infection·2014

This study characterizes brucellaphage, a virus infecting Brucella bacteria. Researchers determined its optimal growth conditions, stability, and key biological and physical characteristics for potential applications.

Area of Science:

  • Microbiology
  • Virology
  • Bacteriology

Background:

  • Brucellaphage is a virus that infects Brucella bacteria, a significant pathogen.
  • Understanding phage characteristics is crucial for developing phage-based therapies and diagnostics.

Purpose of the Study:

  • To characterize a Russian-origin brucellaphage grown on Brucella abortus R 19.
  • To determine optimal propagation methods, stability, and physical properties of the brucellaphage.

Main Methods:

  • Phage propagation using single plaque transfer and optimization of culture conditions (medium, temperature, aeration).
  • Stability testing across various pH levels, temperatures, and in the presence of chloroform.
  • Determination of adsorption rate, latent period, and burst size.

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  • Electron microscopy for morphological analysis.

    • Main Results:

    • Optimal propagation achieved on young Brucella abortus R 19 cultures, yielding titers of approximately 10(10) particles/ml.
    • Phage retained activity after lyophilization and storage for 20 months at 4°C.
    • Phage demonstrated stability between pH 6-8 for 24 hours at 37°C, with some activity loss at 60°C and complete loss with 10% chloroform.
    • Characterized by a slow adsorption rate (K = 3.6 x 10(-11) ml/min), a latent period of 100 min, and a burst size of 121 particles.
    • Electron microscopy revealed a polygonal phage approximately 65 mmu in diameter with a short tail.

    Conclusions:

    • Brucellaphage can be effectively propagated and preserved using established microbiological techniques.
    • The characterized physical and biological properties provide a foundation for its use in research and potential therapeutic applications against Brucella infections.