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Plaque Development and Induction of Interferon Synthesis by RMC Poliovirus

T C Johnson1, L C McLaren

  • 1Department of Microbiology, University of Minnesota, Minneapolis, Minnesota.

Journal of Bacteriology
|September 1, 1965
PubMed

Insights

Interferon production causes autointerference in RMC poliovirus plaque development. This viral inhibitor synthesis in human amnion cells is blocked by actinomycin D and early guanidine hydrochloride treatment.

Area of Science:

  • Virology
  • Cell Biology

Background:

  • Plaque development is crucial for viral quantification.
  • Autointerference can affect viral plaque assays.
  • Interferon is a key antiviral cytokine.

Purpose of the Study:

  • Investigate RMC poliovirus plaque development.
  • Determine the cause of autointerference.
  • Analyze interferon synthesis regulation in infected cells.

Main Methods:

  • Human amnion and HeLa cell monolayers were used for plaque assays.
  • Acid-agar overlay and NaCl extraction were employed.
  • Plaque reduction assays assessed interferon activity.
  • Actinomycin D and guanidine hydrochloride were used to inhibit interferon synthesis.

Main Results:

  • Acid-agar overlay inhibited RMC poliovirus, indicating attenuation (d(-) marker).
  • An agar inhibitor affected plaque development on HeLa cells, removable by NaCl extraction.
  • Interferon production was identified as the cause of autointerference.
  • Interferon synthesis was inhibited by actinomycin D and early guanidine hydrochloride treatment, but not by cell age or chorionic fibroblasts.

Conclusions:

  • Interferon production is responsible for RMC poliovirus autointerference.
  • Specific inhibitors like actinomycin D and guanidine hydrochloride can modulate interferon synthesis.
  • These findings contribute to understanding poliovirus-host interactions and antiviral responses.

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