Related Experiment Video
Updated: Aug 9, 2026
IR-TEx: An Open Source Data Integration Tool for Big Data Transcriptomics Designed for the Malaria Vector Anopheles gambiae
Published on: January 15, 2020
[Cloning, location and differential analysis of transcription factor Relish gene from Anopheles stephensi under
1Department of Pathogen Biology, The Third Military Medical University, Chongqing, China.
Objective:
To clone, locate and differentially analyze the transcription factor Relish gene from Anopheles stephensi, and to examine its signals-modulating action on prophenoloxidase cascade and melanization of Plasmodium yoelii oocysts.
Methods:
Relish cDNA of total mosquitoes was amplified by RT-PCR with degenerated primers. Target PCR product was purified, cloned, sequenced and identified. Special Relish gene was amplified with specific primers from hemocytes or midgut, respectively. Semi-quantitative analysis was made under different feeding conditions. Relish message ribonucleic acid was identified with hybridization in situ.
Results:
One cDNA segment of Relish similar to An. gambiae was acquired from An. stephensi. The same Relish gene was also manifested in the hemocytes and midgut. Marked up-regulation expression of Relish was observed at 6, 12, 24 or 48 h of Plasmodium yoelii infection and at 12 and 24 h after sucking nitroquine-acetate sucrose solution, that was before inducible oocyst melanization. Relish was also expressed in the hemocytes and midguts by ISH.
Conclusion:
Transcription factor Relish of An. stephensi might play a role in signal modulation of Plasmodium yoelii infection and oocyst melanization.
