Related Experiment Video
Updated: Aug 9, 2026
Isolation, Culture, and Adipogenic Induction of Stromal Vascular Fraction-derived Preadipocytes from Mouse Periaortic Adipose Tissue
Published on: July 21, 2023
[Primary culture of human omental preadipocytes]
Zhi-feng Sheng1, Ping-fang Wang, Wei Wang
1Institute of Metabolism and Endocrinology, Second Xiangya Hospital, Central South University, Changsha 410011, China. szf1976@hotmail.com
Objective:
To establish a primary culture method of human omental preadipocytes.
Methods:
Using enzyme-digesting method, fibroblast-like cells from the human omental adipose tissues were cultured, and then differentiated by conditional medium, and identified by oil red O staining.
Results:
The cultured cells were highly homogeneous, and highly proliferative in 4-5th generation. During the process of induction by conditional medium, the cells became round-like and larger, and more adipose droplets were aggregated. By oil red O staining, we confirmed the differentiated cells were mature adipocytes.
Conclusion:
In human omental adipose tissues, there are some preadipocytes, which can differentiate into mature adipocytes with appropriate stimulus.
