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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Sylviside, a diterpene glucoside derivative from Gnaphalium sylvaticum
Mira M Konopleva1, Irena Matławska, Małgorzata Wojcińska
1Department of Pharmacognosy and Botany, Vitebsk State University, Republic of Belarus.
Abstract:
A new diterpene glucoside (1), named sylviside, was isolated from the aerial parts of Gnaphalium sylvaticum. Its structure was elucidated as 2beta,15alpha,20alpha-trihydroxy-19,20-dicarboxy-ent-kaur-16-ene 2beta-O-(2'-angelate)-beta-D-glucopyranoside, on the basis of spectroscopic analysis ((1)H NMR, (13)C NMR, HMQC, HMBC, NOESY), and was confirmed by X-ray crystallographic analysis. Sylviside (1) displayed weak cytotoxicity against HeLa WT (human epitheloid cervical carcinoma) cells and was also evaluated for its effects on reversing multidrug resistance in HeLa cells overexpressing MDR1.
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