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Updated: Aug 9, 2026

The Lambda Select cII Mutation Detection System
Published on: April 26, 2018
Comparative study between the Light Cycler and the PCR-restriction fragment length polymorphism in detecting factor V
Layla Mammo1, Atia Sheereen, Tanya Saour
1Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Center, PO Box 3354, Riyadh 11211, Saudi Arabia. mammo@kfshrc.edu.sa
Objectives:
To test reproducibility, speed and cost of testing for factor V Leiden and FII 20210G>A in our practice.
Design And Methods:
We compared conformity, reproducibility, speed and cost using the Light Cycler (LC) and PCR-RFLP.
Results:
There was 100% conformity and reproducibility. LC was faster but 23% more expensive per sample. When equipment depreciation and patient expenses are added, LC testing becomes cheaper.
Conclusion:
In our practice, LC provides fast, reproducible and cost-effective results.
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