Nanocrystalline diamond modified gold electrode for glucose biosensing

Area of Science:

• Electrochemistry
• Biosensors
• Nanomaterials

Background:

• Boron-doped diamond is common in electrochemical sensors, but non-doped diamond has limited use due to poor conductivity.
• Developing sensitive and selective glucose biosensors is crucial for diagnostics and monitoring.

Purpose of the Study:

• To develop a glucose biosensor using electrochemically pretreated non-doped nanocrystalline diamond (N-NCD) on a gold electrode.
• To investigate the effect of anodic pretreatment on N-NCD conductivity and its application in glucose detection.

Main Methods:

• Coating a gold electrode with N-NCD.
• Immobilizing glucose oxidase (GOx) onto N-NCD via amide linkages.
• Performing anodic electrochemical pretreatment on the N-NCD modified electrode.
• Detecting glucose by monitoring the current change of oxygen reduction at negative potentials.

Main Results:

• Electrochemical pretreatment significantly improved electron transfer rate and oxygen reduction in N-NCD.
• The biosensor demonstrated selective glucose detection in the presence of common interferents like ascorbic acid, acetaminophen, and uric acid.
• Achieved a wide linear calibration range (10 µM to 15 mM) and a low detection limit (5 µM) for glucose.

Conclusions:

• Electrochemical pretreatment is an effective strategy to enhance the performance of non-doped nanocrystalline diamond-based biosensors.
• The developed N-NCD/Au biosensor offers a sensitive and selective platform for glucose detection.
• This approach provides a promising method for electrochemical glucose sensing applications.