Mass spectrometry of macromolecular assemblies: preservation and dissociation
Justin L P Benesch1, Carol V Robinson
1Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.
Abstract:
Mass spectrometry not only plays a crucial role in the identification of proteins involved in the intricate interaction networks of the cell, but also is increasingly involved in the characterization of the non-covalent complexes formed by interacting partners. Recent developments have enabled the use of gas phase dissociation to probe oligomeric organization and topology, and increased understanding of the electrospray process is leading to knowledge of the structure of protein assemblies both in solution and in the gas phase.
Related Concept Videos
Tandem Mass Spectrometry
Mass Spectrometry: Overview
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
MALDI-TOF Mass Spectrometry
Mass Spectrometers


