Selection and identification of bacteria isolated from waste crude oil with polycyclic aromatic hydrocarbons removal capacities
Related Experiment Video
Updated: Aug 9, 2026

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
Published on: December 7, 2021
F L Toledo1, C Calvo, B Rodelas
1Group of Environmental Microbiology, Department of Microbiology, Institute of Water Research, University of Granada, C/Ramón y Cajal n 4, Edificio Fray Luis de Granada, 18071 Granada, Spain.
Abstract:
Fifteen bacterial strains isolated from solid waste oil samples were selected due to their capacity of growing in the presence of hydrocarbons. The isolates were identified by PCR of the 16S rDNA gene using fD1 and rD1 primers. The majority of the strains belonged to genera Bacillus, Bacillus pumilus (eight strains) and Bacillus subtilis (two strains). Besides, three strains were identified as Micrococcus luteus, one as Alcaligenes faecalis and one strain as Enterobacter sp. Growth of the above-mentioned strains in mineral liquid media amended with naphthalene, phenanthrene, fluoranthene or pyrene as sole carbon source was studied and our results showed that these strains can tolerate and remove different polycyclic aromatic hydrocarbons that may be toxic in the environment polluted with hydrocarbons. Finally, the capacity of certain strains to emulsify octane, xilene, toluene, mineral oil and crude oil, and its ability to remove hydrocarbons, look promising for its application in bioremediation technologies.
More Related Videos
Related Concept Videos
Microbial Bioremediation of Hydrocarbons
Bioremediation
Microbial Bioremediation of Plastics

