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Monitoring bovine viral diarrhea virus vaccines for adventitious virus, using T1 ribonuclease viral RNA
C L Kelling1, J E Kennedy, K K Rump
1Department of Veterinary Science, University of Nebraska, Lincoln 68583-0905.
American Journal of Veterinary Research
|August 1, 1991
Abstract:
Viral RNA oligonucleotide fingerprinting was used to discriminate 3 cytopathic vaccine bovine viral diarrhea viruses (BVDV) grown in medium supplemented with serum contaminated with noncytopathic BVDV from the same 3 viruses grown in cell culture free of BVDV. Oligonucleotide fingerprinting also effectively discriminated between reference Singer BVDV, NADL BVDV, and New York-1 BVDV grown in BVDV-free noncontaminated or BVDV-contaminated cell cultures. Oligonucleotide fingerprint mapping of viral RNA maybe used to determine the purity of virus stocks, as well as that of BVDV vaccines.