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Updated: Aug 9, 2026
A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
Published on: April 20, 2018
Silver staining method for DNA in polyacrylamide gels using eriochrome black T as a silver-ion sensitizer
Sun-young Hwang1, Li-tai Jin, Gyurng-soo Yoo
1College of Pharmacy, Chonnam National University, Buk-ku, Kwangju, South Korea.
Abstract:
A sensitive silver staining method using eriochrome black T as a silver-ion sensitizer for DNA detection in polyacrylamide gels was developed. The sensitivity of this staining method was significantly improved by the new silver-ion sensitizer containing a diazo group, which has reducing power. The staining method lasted a total of approximately 15 min following a fixing step for 2 x 20 min. The detection limit of this staining method was 1-4 pg for PhiX174 DNA/HaeIII in both nondenaturing and denaturing polyacrylamide gels. This staining method was especially effective in low-base pair DNA, with a sensitivity that was approximately ten-fold higher than previously published silver staining methods.
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