[Active nitrogen and oxygen metabolites change cAMP level in the uterus myocytes treated by progesterone]

The level of cAMP in the rats uterus myocytes under the effect of active metabolites of nitrogen and oxygen (NO, NO2- and H2O2) was studied in the conditions of progesterone influence on myocytes. Suspension of cells was selected with the use of collagenase and soy-bean inhibitor of tripsine. The amount of cAMP was determined with the use of standard reagents producted by "Amersham", England. It is established that the basal level of cAMP in cells is 10.4 +/- 0.7 pmol cAMP/mg protein. Incubation of myocytes with forskoline, 0.1 mM, 10 min, resulted in its 3.4 times increase. It testifies to adenilatcyclase activity characteristic of the great majority of cell objects in the uterus myocytes. The level of cAMP in the suspension insignificantly decreases at the protracted affecting with myocytes 10 nM progesterone (1 hour). Donator of nitrogen oxide (0.1 mM sodium nitroprusside) in the presence of progesterone substantially promoted the level of cAMP in cells at the protracted action (17 +/- 3 pmol cAMP/mg protein). Nitrite-anion and hydrogen peroxide in concentration 10 nM (low physiological concentration) did not have the above effect. The results obtained prove that exactly the long-term influence of nitrogen oxide, instead of progesterone, can provide the increase of cAMP level in the uterus myometrium.