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[Effect of Herba Epimedii Flavone on expression of OPG and RANKL in rat osteoblasts]
Yiheng Liu1, Hongmin Zang, Haiying Zhang
1Department of Orthopedics, Second Hospital of Xi' an Jiaotong University, Xi' an.
Objective:
To explore the effect of Herba Epimedii Flavone (HEF) on expression of OPG and RANKL in rat osteoblasts.
Methods:
Osteoblasts were obtained from new born rat calvaria by digestive enzymes. The different HEF concentrations (0.1, 1, 10, 100 ng/ml)were added into the culture medium. Total mRNA was prepared from osteoblasts after 48 h and 96 h, and mRNA expression of OPG and RANKL were detected by RT-PCR.
Results:
The mRNA expression of OPG in osteoblasts significantly increased compared with that of the control group, and the V(OPF)/V(RANKL) ratio was deceased markedly in the treatment of 10 ng/ml group after 48 h. After 96 h, the mRNA expression of OPG significantly increased and the V(OPG)/V(RANKL) ratio was also decreased in the treatment group compared with that of the control group.
Conclusion:
HEF suppress the differentiation and maturate of osteoblast. Osteoporosis can be prevented and cured by HEF.