Related Experiment Video
Updated: Aug 9, 2026

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Chelating properties and molecular weight distribution of soluble microbial products from an aerobic membrane
Ladan Holakoo1, George Nakhla, Ernest K Yanful
1Department of Chemical and Biochemical Engineering, University of Western Ontario, London, Ont, Canada N6A 5B9.
Abstract:
This paper presents a detailed study on soluble microbial products (SMPs) in an aerobic membrane bioreactor (MBR) treating synthetic wastewater simulating municipal wastewater. The concentration of SMP in the reactor conformed to a cyclical pattern of accumulation and reduction in relation to SRT. The molecular weight (MW) distribution of accumulated SMP was determined to vary from <1kD to >100kD. Copper chelating properties of various SMP fractions in the MBR were compared before and after copper addition to the feed. The conditional stability constant (LogcK), complexation capacity (Cc), and SMP-ligand concentration (CL) were evaluated to determine the impact of copper on the chelating properties. The results indicated that accumulated SMP in the aerobic MBRs without copper addition are moderate chelators with LogcK values of 7.6-8.3 mol(-1) for the moderate ligands and 6.3-6.8 mol(-1) for the relatively weaker ligands. SMPs with MW of 1-10 kD were found to have the highest complexation capacity among all SMP fractions. The complexation capacity of accumulated SMP after feeding copper was 0.11 micromol/mg of SMP, almost half of its value prior to feeding copper. The reduction of C(c) after feeding copper was a result of an increase in large molecular weight SMP (>100 kD).
Related Concept Videos
Detergent Purification of Membrane Proteins
Factors Affecting Solubility
Bioreactor Design and Operational System
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...

