Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Exploring human plasma proteome strategies: high efficiency in-solution digestion protocol for multi-dimensional

Qinhua Cindy Ru1, Luwang Andy Zhu, Richard A Katenhusen

  • 1Windber Research Institute, PA 15963, USA. c.ru@wriwindber.org

Journal of Chromatography. A
|March 30, 2006
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Examining Cost Differences in Malignant Brain and Central Nervous System Tumor Treatment Across US Military Health System Care Settings.

Journal of healthcare management / American College of Healthcare Executives·2026
Same author

Chronic inflammatory diseases and survival among patients with pancreatic cancer in the U.S. Military health system.

The American journal of medicine·2026
Same author

The Development of Tools to Investigate Potential Sources of Sample Misidentification Within a Biorepository: Case Studies.

Biopreservation and biobanking·2026
Same author

Incidence of Hodgkin and Non-Hodgkin Lymphomas: Comparison of the Military and General Population.

Cancer medicine·2026
Same author

The relationship between asthma and glioma: a case-control study in a universal access healthcare system.

Journal of neuro-oncology·2026
Same author

Age Comparisons of Endometrial Cancer Clinicopathology and Treatment in the United States Military Health System.

Military medicine·2026

This study compared three in-solution digestion protocols for multi-dimensional protein identification technology (MudPIT). Results show that using trifluoroethanol (TFE) as a denaturant provides the most efficient protein digestion for complex proteomic samples.

Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Multi-dimensional protein identification technology (MudPIT) is a high-throughput proteomic method.
  • Complex samples require efficient in-solution digestion prior to MudPIT analysis.
  • Evaluating the efficiency of in-solution digestion protocols is crucial for proteomic studies.

Purpose of the Study:

  • To compare the efficiency of three different in-solution digestion protocols.
  • To identify the most effective protocol for preparing complex samples for MudPIT.

Main Methods:

  • Comparison of three distinct in-solution digestion protocols.
  • Assessment of digestion efficiency for complex protein mixtures.
  • Utilized multi-dimensional protein identification technology (MudPIT) for analysis.

Related Experiment Videos

Main Results:

  • One protocol demonstrated superior efficiency compared to the others.
  • The protocol employing trifluoroethanol (TFE) as a denaturant was found to be the most efficient.
  • Efficient digestion is critical for comprehensive protein identification in MudPIT.

Conclusions:

  • Trifluoroethanol (TFE) is an effective denaturant for optimizing in-solution protein digestion.
  • The choice of digestion protocol significantly impacts the efficiency of MudPIT analysis.
  • Further research into optimizing sample preparation for proteomics is warranted.