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Related Experiment Videos

Organization of tn2610 containing two transposition modules.

Akiko Takaya1, Masato Watanabe, Tomoko Yamamoto

  • 1Department of Microbiology and Molecular Genetics, Graduate School of Pharmaceutical Sciences, Chiba University, Chiba, 263-8522, Japan.

Antimicrobial Agents and Chemotherapy
|March 30, 2006
PubMed
Summary

The transposon Tn2610, identified in hospital-acquired Escherichia coli, exhibits a complex mosaic structure. Its sequencing reveals a hybrid origin from Tn1721 and Tn21 elements, incorporating multiple resistance genes.

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Area of Science:

  • Microbiology
  • Genomics
  • Molecular Biology

Background:

  • Transposons are mobile genetic elements that play a significant role in bacterial evolution and the spread of antibiotic resistance.
  • Understanding the structure and origin of complex transposons like Tn2610 is crucial for tracking antimicrobial resistance mechanisms.

Purpose of the Study:

  • To completely sequence and characterize the transposon Tn2610.
  • To elucidate the structural organization and potential evolutionary origins of Tn2610.

Main Methods:

  • Whole-genome sequencing of the conjugative plasmid carrying Tn2610.
  • Bioinformatic analysis to identify genetic modules, genes, and structural features of Tn2610.

Main Results:

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  • Tn2610 is 23,883 bp long, composed of Tn1721-like and Tn21-derived modules (IRa and IRb).
  • Functional analysis revealed that only the tnpA gene in the Tn21-derived module (IRb) is active in transposition.
  • The transposon contains multiple integron-associated resistance genes within a Tn21 backbone, including a region shared with Salmonella enterica serovar Typhimurium DT104.

    • Conclusions:

    • Tn2610 likely originated from recombination between Tn1721 and Tn21 elements, with subsequent integration of other genetic elements.
    • The complex mosaic structure of Tn2610 highlights the dynamic nature of transposon evolution and the potential for rapid dissemination of antibiotic resistance genes.