Related Experiment Video

Updated: Aug 9, 2026

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method
12:03

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method

Published on: April 18, 2019

Novel carbapenem-hydrolyzing oxacillinase OXA-62 from Pandoraea pnomenusa

Ines Schneider1, Anne Marie Queenan, Adolf Bauernfeind

  • 1MICOER Institute, Hesseloherstrasse 4, 80802 Munich, Germany.

Antimicrobial Agents and Chemotherapy
|March 30, 2006
PubMed

Insights

A novel carbapenem-hydrolyzing oxacillinase, OXA-62, was discovered in Pandoraea pnomenusa, an antibiotic-resistant bacterium. This finding complicates treatment for cystic fibrosis patients infected with this species.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Antimicrobial Resistance

Background:

  • Pandoraea spp. are gram-negative bacteria found in cystic fibrosis patients.
  • These bacteria exhibit resistance to multiple antibiotic classes, with limited treatment options.

Purpose of the Study:

  • To investigate the mechanism of imipenem resistance in a Pandoraea pnomenusa strain.
  • To identify and characterize novel beta-lactamases responsible for carbapenem resistance.

Main Methods:

  • Isolation and characterization of Pandoraea pnomenusa.
  • Cloning and sequencing of beta-lactamase genes.
  • Determination of beta-lactamase activity against various antibiotics.

Main Results:

  • Identification of two beta-lactamases: OXA-33 and a novel carbapenem-hydrolyzing oxacillinase, OXA-62.
  • OXA-62 confers resistance to penicillins, oxacillin, imipenem, and meropenem.
  • The blaOXA-62 gene is chromosomally located and appears specific to P. pnomenusa strains.

Conclusions:

  • OXA-62 represents a new mechanism of carbapenem resistance in Pandoraea pnomenusa.
  • The presence of OXA-62 complicates treatment strategies for P. pnomenusa infections.
  • Further research is needed to understand the prevalence and clinical impact of OXA-62.

More Related Videos

Antibiotic Dereplication Using the Antibiotic Resistance Platform10:49

Antibiotic Dereplication Using the Antibiotic Resistance Platform

Published on: October 17, 2019

Related Concept Videos

Antibiotic Selection00:57

Antibiotic Selection

Overview
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Production of Antibiotics01:27

Production of Antibiotics

Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...