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Differential effects of propofol and ethanol on P2X4 receptors expressed in Xenopus oocytes
D L Davies1, S T Kuo, R L Alkana
1Department Molecular Pharmacology and Toxicology, School of Pharmacy, University of Southern California, Los Angeles, CA United States.
Abstract:
The current study investigated the effects of propofol on P2X4 receptors expressed in Xenopus oocytes using two-electrode voltage clamp. We also tested the effects of 100 mM ethanol on the same oocytes used to test propofol. Propofol potentiated ATP-gated currents in a concentration dependent manner in P2X4 receptors. In agreement with our previous findings, ethanol inhibited P2X4 receptors. The opposite effects of propofol and ethanol on P2X4 receptor function suggest that these anesthetics act via different sites/mechanisms in P2X receptors as has been suggested for GABA(A) and glycine receptors.
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