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[Karyotypes analysis of Ferula fukanensis]
Xin Zhao1, Xiao-Jun Ma, Sulaiman Kaisar
1Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100094, China.
Objective:
Study on the karyotypes analysis of Ferula fukanensis.
Method:
The young roots were treated with 0.000 2 mol x L(1) 8-Hydroxyquinoline for 3 h, carnoy's for 3 h, 1 mol x L(-1) HCl in 5 min,carbol fuchsin coloration for 2 min and the treated roots were utilizied to make the plate for observation.
Result:
The fukanensis is diploid. The chromosome number of somatic cells was 2n = 22. The karyotype formula is 2n = 2x = 20 = 16m + 4sm. The 4th and 10th are submetacentric, and the others are metacentric.
Conclusion:
The karyotype of F. fukanensis belongs to "2A" type of stebbins', and it is a primitive species.
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