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Updated: Aug 9, 2026

13C6-Glucose Labeling Associated with LC-MS: Identification of Plant Primary Organs in Secondary Metabolite Synthesis
Published on: March 22, 2024
[Studies on chemical constituents of Patrinia villosa]
Jin-Yong Peng1, Guo-Rong Fan, Yu-Tian Wu
1College of Pharmacy, Second Military Medical University, Shanghai 200433, China.
Objective:
To investigate the chemical constituents of Patrinia villosa.
Method:
The chemical constituents were isolated by silica gel column chromatography and semi-preparative high-performance liquid chromatography, and identified by physicochemical properties and spectral analysis (MS, 1H-NMR and 13C-NMR).
Result:
Seven compounds were isolated from ethyl acetate and n-butanol extract and identified as: 5-hydroxyl-7, 3', 4'-trimethoxy flavone (I), 5-hydroxyl-7, 4'-dimethoxy flavone (II), luteolin (III), quercetin (IV), isoorientin (V), isovitexin (VI) and 8-C glucosylprunetin (VII).
Conclusion:
Compounds I , II, III, V, VI and VIII were obtained from the plant of genus Patrinia for the first time, compound IV was separated from P. villosa for the first time.
