Related Experiment Video
Updated: Aug 9, 2026

2D-HPLC-MS Technology Combined with Molecular Network for the Identification of Components in Tibetan Medicine Aconitum pendulum
Published on: December 8, 2023
[Chemical constituents from root of Lasianthus acuminatissimus I]
Bin Li1, Dong-Ming Zhang, Yong-Ming Luo
1Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China.
Objective:
To investigate the chemical constituents from the root of Lasianthus acuminatissimus of Rubiaceae.
Method:
Normal, reverse phase silica gel, macroporous resin column and HPLC chromatography were used for isolation. Spectroscopic methods (13C-NMR, 1H-NMR, DEPT, EI-MS) were used for identification.
Result:
Six compounds were isolated and elucidated as isoscopletin(I), isoscopletin-6-O-beta-D-glucopyranoside (II), beta configuration of Mudanoside-A (Ill), asperuloside (IV), (-)-pinoresinol 4-O-beta-D-glucopyranoside (V), daucosterol (VI).
Conclusion:
These compounds were obtained from this plant for the first time.